Molecular characterization, expression, polymorphism of NR5A2 and its relationship with litter size in Hu sheep

YX Li, J Zhang, Y Qian, CH Meng… - Genetics and …, 2015 - funpecrp.com.br

… Amplification products were separated and visualized by agarose gel electrophoresis, and purifiedwith a DNA gel extraction kit (Karroten, Nanjing, China). Recovered DNA fragments were ligatedinto pMD18-T vectors and transformed into Escherichia coli DH5α …

 

Molecular Characterization and Expression of PFT, an FHB Resistance Gene at the Fhb1 QTL in Wheat

Y He, X Zhang, Y Zhang, D Ahmad, L Wu… - …, 2018 - Am Phytopath Society

… Genomic DNA was extracted from the fresh leaves of 348 wheat accessions by using Karroten Plant Genomic DNA Extraction Kit (Karroten). The RNA and DNA quality and quantity weredetermined by agarose gel electrophoresis and NanoDrop 2000 (Thermo Scientific) …

 

Mitochondrial genome is paternally inherited in Cucumis  allotetraploid (C.× hytivus) derived by interspecific hybridization

J Shen, MG Kere, JF Chen - Scientia Horticulturae, 2013 - Elsevier

… after staining with ethidium bromide. 2.3. Cloning and sequences analysis. The PCR
products of the expected size were recovered and purified using a DNA Gel Extraction
Kit (Karroten, China). The purified fragments were cloned …

 

Genetic diversity of Ty1-copia retrotransposons in a wild species of Cucumis (C. hystrix)

B Jiang, ZM Wu, QF Lou, D Wang, WP Zhang… - Scientia horticulturae, 2010 - Elsevier

… 2.3. Cloning and sequencing. The PCR products of expected size were recovered and purifiedby DNA Gel Extraction Kit (Karroten, China). The purified fragments were cloned into the vectorpMD19-T (Takara, Japan) and then transformed into E. coli strain DH5α …

 

Retrotransposon- and microsatellite sequence-associated genomic changes in early generations of a newly synthesized allotetraploid Cucumis × hytivus Chen & …

B Jiang, Q Lou, Z Wu, W Zhang, D Wang… - Plant molecular …, 2011 - Springer

… DNA fragments with altered patterns between parental lines and the allotetraploid (parental bandsabsent in the allotetraploid or novel bands in the allotetraploid) were excised from the gel, purifiedwith a DNA gel extraction kit (Karroten, China), cloned into the pMD19-T vector …

 

 Diversity of endophytic fungi of Myricaria laxiflora grown under pre-and post-flooding conditions

W Tian, YH Bi, W Zeng, W Jiang, YH Xue… - Genet Mol …, 2015 - geneticsmr.com

… Following morphologic observation and microscopic examination, ITS sequence analysis wascarried out on each isolate. Total genomic DNA was extracted from cultures at 7-14 days, usinga microbe genomic DNA isolation kit (Karroten Life Scientific, China) …

Transcription profiles of genes related to hormonal regulations under salt stress in sweet sorghum

Z Yang, Y Wang, X Wei, X Zhao, B Wang… - Plant Molecular Biology …, 2017 - Springer

… Total RNA was isolated from the leaves and seminal roots of each genotype treated with 0 and150 mM NaCl for 48 h. Total RNA isolation was performed using a Total Plant RNA Extraction Kit (Karroten, Beijing, China) following the manufacturer's protocols …

 

Transcriptome analysis of sweet Sorghum inbred lines differing in salt tolerance provides novel insights into salt exclusion by roots

Z Yang, H Zheng, X Wei, J Song, B Wang, N Sui - Plant and Soil, 2018 - Springer

… Total RNA extraction Total RNA was isolated from the primary roots of each genotype treated with 0 and 150 mM NaCl for 48 h using a Total Plant RNA Extraction Kit (Karroten, Bei- jing, China) following the manufacturer's protocols as described previously (Sui et al. 2015) …

 

Ultra performance liquid chromatography/ quadrupole-time -of-flight mass spectrometry for determination of avicularin metabolites produced by a human intestinal …

M Zhao, J Xu, D Qian, J Guo, S Jiang, E Shang… - …Journal of Chromatography B, 2014 - Elsevier… 2.7. 16S rRNA gene sequencing and phylogenetic analysis. The genomic DNA of strain 46 wasextracted from the isolate using a Karroten Genomic DNA Purification kit (Karroten, China) … ThePCR product was purified from the agarose gel using a Karroten gel purification kit …

 

Application of ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry to determine the metabolites of orientin produced by …

J Xu, D Qian, S Jiang, J Guo, E Shang, J Duan… - … of Chromatography B, 2014 - Elsevier

… 2.7. 16S rRNA gene sequencing and phylogenetic analysis. Genomic DNA was extracted fromthe isolate using a Karroten genomic DNA purification kit (Karroten, China) … The PCR product was purified from the agarose gel using a Karroten gel purification kit …

 

 

The transcriptome of NaCl-treated Limonium bicolor leaves reveals the genes controlling salt secretion of salt gland

F Yuan, MJA Lyu, BY Leng, XG Zhu, BS Wang - Plant molecular biology, 2016 - Springer

… sequencing analysis. The total RNA was extracted using a Total Plant RNA Extraction
Kit (Karroten, Beijing, China) and purified with an RNeasy Mini RNA kit (Qiagen,
Germany) including a one-column DNase digestion. The …

 

Comparative transcriptome analysis of developmental stages of the Limonium bicolor leaf generates insights into salt gland differentiation

F Yuan, MJUAMY LYU, BY LENG… - Plant, cell & …, 2015 - Wiley Online Library

… Total RNA from different samples was extracted using a Total Plant RNA Extraction
kit (Karroten, Beijing, China) and purified using the RNAeasy Mini RNA kit (Qiagen,
Shanghai, China) including on-column DNase digestion …

 

LTR retrotransposons cause expression changes of adjacent genes in early generations of the newly formed allotetraploid Cucumis hytivus

L Jia, Q Lou, B Jiang, D Wang, J Chen - Scientia Horticulturae, 2014 - Elsevier

… ethidium bromide. The expected fragments were recovered by DNA Gel Extraction
Kit (Karroten, China) and then ligated into the pMD19-T vector (TaKaRa, Japan) and
transformed into Escherichia coli strain DH5α. Recombinant …

 

Physiological and molecular evidence for Na+ and Cl− exclusion in the roots of two Suaeda salsa populations

Q Liu, R Liu, Y Ma, J Song - Aquatic Botany, 2018 - Elsevier

… Total RNA was extracted from roots of S. salsa from the intertidal zone and inland saline soils(a 0.2 g subsample per biological replicate) using a Total Plant RNA Extraction Kit (Karroten,Beijing, China) following the manufacturer's protocols (Sui et al., 2015) …

 

 

The interactions between gut microbiota and entomopathogenic fungi: a potential approach for biological control of Blattella germanica (L.)

F Zhang, XX Sun, XC Zhang, S Zhang… - Pest management …, 2018 - Wiley Online Library

… 2.8 PCR amplification and pyrosequencing. Gut homogenates from 60 cockroaches
were prepared and DNA was extracted using the K2306 Karroten Microbial Genomic
DNA extraction kit (Novoprotein Scientific Inc., Shanghai, China) …

 

NADP-malate dehydrogenase of sweet sorghum improves salt tolerance of Arabidopsis thaliana

Y Guo, Y Song, H Zheng, Y Zhang… - Journal of agricultural …, 2018 - ACS Publications

… 127 Cloning the sequence of SbNADP-ME 128 Total RNA was isolated from leaves of sweetsorghum using the Total Plant RNA Extraction Kit 129 (Karroten 1103) in accordance withthe manufacturer's instructions. The full-length NADP-ME gene 130 …

 

Transcriptome profiling of genes involved in photosynthesis in Elaeagnus angustifolia L. under salt stress

J Lin, JP Li, F Yuan, Z Yang, BS Wang, M Chen - Photosynthetica - Springer

… RNA-Seq analysis: Total RNA was extracted from the leaves of E. angustifolia seedlings
treated with 0 or 150 mM NaCl for 48 h using the Total Plant RNA Extraction Kit (Karroten,
Beijing, China) following the manufacturer's protocol …

 

 

[HTML] Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy …

Z Zhao, L Zhang, XD Guo, LL Cao, TF Xue… - Frontiers in molecular …, 2017 - frontiersin.org

… Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR). Total RNA wasisolated from brain tissues, cell samples, or cultured cells using TRIzol reagent (Karroten) andprepared for quantitative reverse transcriptase PCR using Master Mix (TaKaRa, Japan) …

 

[HTML] Overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis

N Sui, S Tian, W Wang, M Wang, H Fan - Frontiers in plant science, 2017 - frontiersin.org

… determined 14 days later. Cloning and Sequencing of SsGPAT. Total RNA was isolated
from the leaves of S. salsa using a Total Plant RNA Extraction Kit (Karroten 1103)
according to the manufacturer's instructions. In the experiment …

 

A system for the transformation and regeneration of the recretohalophyte Limonium bicolor

F Yuan, M Chen, J Yang, B Leng, B Wang - In Vitro Cellular & …, 2014 - Springer

… Total RNA from the uninfected and infected regenerated plantlets was extracted using the TotalPlant RNA Extraction Kit (Karroten, Beijing, China) and then first-strand complementary DNAs(cDNAs) were synthesized from DNaseI-treated total RNA using ReverTra Ace qPCR …

 

Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and …

XJ Zhao, Z Zhao, DD Yang, LL Cao, L Zhang, J Ji… - Brain research …, 2017 - Elsevier

… qRT-PCR). The hypothalamus was separated from the frozen brain. Total RNA was
extracted by using a TRIzol reagent (Karroten) and prepared for quantitative reverse
transcriptase PCR by using Master Mix (TaKaRa, Japan) …

 

Gene-indexed mutations in maize

X Lu, J Liu, W Ren, Q Yang, Z Chai, R Chen, L Wang… - Molecular plant, 2018 - Elsevier

… plants. Construction of Exome Sequencing Libraries. Genomic DNA was isolated
from young leaves of 1090 mutated plants in the M 1 generation using the Plant
Genomic DNA Extraction Kit (Karroten, Nanjing, Jiangsu, China) …

 

 [PDF] NaCl, 蔗糖和KH2PO4 對(duì)紅葉桃離體葉片花色苷合成因表達(dá)的影響

文楊， 韓鍵， 姜衛(wèi)兵， 王小青， 房經(jīng)貴， 張斌斌， 馬瑞娟 - 園藝學(xué)報(bào), 2016 - ahs.ac.cn

… 按照植物總RNA 提取試劑盒Karroten產(chǎn)品說(shuō)明書(shū)進(jìn)行桃葉片RNA
的提取，并用TaKaRa Recombinant DNase I（RNase-free）去除RNA 中的DNA。提取的RNA 立即進(jìn)行反轉(zhuǎn)錄，或保存在–80 ℃冰箱備用。 以提取的總RNA 為模板，利用TaKaRa Prime ScriptTM …

 

[PDF] 基于基因組重測(cè)序信息構(gòu)建玉米第5 染色體的片段代換系

林峰， 葛敏， 寶華賓， 趙涵 - 玉米科學(xué), 2015 - ymkx.com.cn

… 將群體所有單株及親本取樣，植物葉片用低溫 真空干燥儀干燥后，采用Karroten 植物基因組DNA提取試劑盒抽提DNA，具體步驟詳見(jiàn)試劑盒說(shuō)明 書(shū)。DNA加適量TE溶解后，4℃或-20℃貯藏待用。PCR反應(yīng)體系為25 μL，包含5 pmol兩側(cè)引物，2.5 μL 10 x buffer，1.25 nmol dNTP，1 U …

 

[PDF] 海濱雀稗體細(xì)胞突變體SP2008-3 的特異性分析

常盼盼， 鐘小仙， 劉智微 - 草業(yè)學(xué)報(bào), 2012 - cyxb.magtech.com.cn

… 拍照和統(tǒng)計(jì)染色體數(shù)目。 １．２．４ ＲＡＰＤ分子鑒定取海濱雀稗體細(xì)胞突變體ＳＰ２００８３、原始對(duì)照Ａｄａｌａｙｄ（ＣＫ１）及其幼穗離體培養(yǎng)再 生植株（ＣＫ２）的幼嫩葉片，采用Karroten公司生產(chǎn)的植物ＤＮＡ提取試劑盒（Karroten Plant Genomic DNA …

 

[HTML] 利用Insertion/Deletion (InDel) 分子標(biāo)記檢測(cè)玉米互交種混雜的原理及應(yīng)用

葛敏， 蔣璐， 張曉林， 趙涵， 張?bào)w付 - 《 分子植物育種》 官方網(wǎng)站, 2012 - biopublisher.cn

… 也適用于具有可用InDel標(biāo)記的其它作物互交種的鑒定。 3材料與方法 3.1材料 供試材料為玉米自交系B73、Mo17、正交種B73×Mo17(BM)及反交種Mo17×B73(MB)。 3.2 DNA提取 B73、Mo17、BM、MB葉片DNA以及5個(gè)BM、5個(gè)MB胚乳樣本DNA提取參照Karroten DNA提取試劑盒，DNA …

 

[PDF] 紫花苜蓿γ-生育酚甲基轉(zhuǎn)移酶(γ-TMT) 基因的克隆與逆境下的表達(dá)分析

賈會(huì)麗， 王學(xué)敏， 高洪文， 董潔， 王運(yùn)琦， 劉建寧… - 草業(yè)學(xué)報(bào), 2012 - cyxb.lzu.edu.cn

… 拍照和統(tǒng)計(jì)染色體數(shù)目。 １．２．４ ＲＡＰＤ分子鑒定取海濱雀稗體細(xì)胞突變體ＳＰ２００８３、原始對(duì)照Ａｄａｌａｙｄ（ＣＫ１）及其幼穗離體培養(yǎng)再 生植株（ＣＫ２）的幼嫩葉片，采用Karroten公司生產(chǎn)的植物ＤＮＡ提取試劑盒（Karroten Plant Genomic DNA  …